Detection of chlamydial inclusions in cell culture or biopsy tissue by alkaline phosphatase-anti-alkaline phosphatase staining
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چکیده
منابع مشابه
Immunocytochemical identification of cells of megakaryocytic lineage by alkaline phosphatase anti-alkaline phosphatase method.
proliferative disorders. It is necessary to accurately identify the megakaryocytic cells in order to diagnose megakaryocytic leukemia and to assess the significance of the pres ence of such cells in the circulating blood . At present, megakaryocytes and their precursors can be best identified either by ultrastructural study of platelet peroxidase or by immunocytochemical studies using monoclona...
متن کاملImproved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence.
AIMS To develop and immunohistochemical staining method for cryostat and paraffin wax sections so that two different antigens in the same section of tissues could be detected by combining immunoenzyme and immunofluorescence techniques. METHODS This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and in...
متن کاملAlkaline Phosphatase in Serum is a Marker of Human Toxocariasis
Background: The present study was carried out on the human population of Kashmir valley to evaluate the status of the biochemical parameters of infected population. Materials and Methods: Blood samples were collected from 514 individuals, 298 (57.97%) males and 216 (42.02%) females, 187 (36.38%) were found sero-positive for human toxocariasis. Results: Alkaline phosphatase level was found highe...
متن کاملRunning title: Alkaline phosphatase inhibition and Francisella survival 1 Francisella DnaK Inhibits Tissue Nonspecific Alkaline Phosphatase
Following pulmonary infection with Francisella tularensis, we observed an unexpected but significant reduction of alkaline phosphatase, an enzyme normally up-regulated following inflammation. However, no reduction was observed in mice infected with a closely related Gram negative pneumonic organism (Klebsiella pneumonia) suggesting the inhibition may be Francisella specific. In similar fashion ...
متن کاملFrancisella DnaK inhibits tissue-nonspecific alkaline phosphatase.
Following pulmonary infection with Francisella tularensis, we observed an unexpected but significant reduction of alkaline phosphatase, an enzyme normally up-regulated following inflammation. However, no reduction was observed in mice infected with a closely related gram-negative pneumonic organism (Klebsiella pneumoniae) suggesting the inhibition may be Francisella-specific. In similar fashion...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1987
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.25.10.1864-1867.1987